PLK1 anticorps (pThr210)

N° du produit ABIN372703

Cet anticorps Lapin Polyclonal détecte spécifiquement PLK1 dans WB. Il présente une réactivité envers Humain, Souris, Rat, Xenopus laevis, Boeuf (Vache), Chien, Poisson zèbre (Danio rerio) et Non-Human Primate.

Aperçu rapide pour PLK1 anticorps (pThr210) (ABIN372703)

Antigène: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivité: Humain, Souris, Rat, Xenopus laevis, Boeuf (Vache), Chien, Poisson zèbre (Danio rerio), Non-Human Primate

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PLK1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-PLK1 anticorps

Épitope: pThr210

Specificité: This antibody recognizes the ~66k PLK protein phosphorylated at Thr210. Immunolabeling of the PLK band is completely blocked by lambda-phosphatase treatment.

Réactivité croisée (Details): Species reactivity (expected):Bovine, Canine, Mouse, Xenopus, Zebrafish and non-Human Primates.
Species reactivity (tested):Rat and Human.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogène: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr210 of PLK1.

Isotype: IgG

Information d'application

Indications d'application: Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C.

Détails sur PLK1

Antigène: PLK1 (Polo-Like Kinase 1 (PLK1))

Autre désignation: PLK1

Sujet: Polo-like kinases are important regulators of cell cycle progression. PLK1 is a highly conserved Ser/Thr kinase that has essential roles in the formation of mitotic bipolar spindles (van Vugt et al., 2004). Deregulated expression of PLK's is detected in many types of cancer and associated with oncogenesis (Takei et al., 2005). It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser137 and Thr210 (van de Weerdt et al., 2005).Synonyms: PLK-1, Polo-like kinase 1, STPK13, Serine/threonine-protein kinase PLK1

ID gène: 5347

NCBI Accession: NP_005021

UniProt: P53350

Pathways: Cycle Cellulaire, M Phase